About That SV40 in mRNA Jabs

Getting all the ducks in a row.

As if we didn’t have enough issues with the Covid “vaccines”, here comes a new revelation making splashes in the metaverse. The Vigilant Fox tweets that:

“🚨 Cancer Virus Found in COVID Shots: “This Is Looking Very Bad,” Says Dr. Peter McCullough. “SV40 is a known cancer-promoting segment of DNA. And yes, they’re in the shots,” reported @P_McCulloughMD. “What I’m telling you is the shots promote cancer through SV40, and they inhibit our ability to fight cancer by suppressing the tumor suppressor system. So now this is looking very bad. Every system is showing cancer rates are up. So, that’s inarguable. The big question is, how much of this is due to the vaccines?”

What is SV40? According to Wikipedia:

SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that sometimes causes tumors in animals, but most often persists as a latent infection. SV40 has been widely studied as a model eukaryotic virus, leading to many early discoveries in eukaryotic DNA replication and transcription.

Very cool! What’s not cool is that 10-30% of the polio vaccines in the US population were SV40-infected [imagine that curve ball!] as of 1960. As this link tells us:

Vaccine contamination with Simian vacuolating virus 40, known as SV40 occurred in the United States and other countries between 1955 and 1961. An analysis presented at the Vaccine Cell Substrate Conference in 2004 suggested that vaccines used in the former Soviet bloc countries, China, Japan, and Africa, could have been contaminated up to 1980, meaning that hundreds of millions more could have been exposed to the virus unknowingly.

Carbone discovered that it contained not only the SV40 strain already known to have been in the Salk vaccine (containing two 72-bp enhancers) but also the same slow-growing SV40 strain currently found in some malignant tumors and lymphomas (containing one 72-bp enhancer).

Take note of (a) one (b) 72bp enhancer - it will play a prominent role in the discussion below. Encouragingly:

A thirty-five year follow-up found no excess of the cancers commonly associated with SV40.[11]

Well, not quite. This “Thirty-five year mortality following receipt of SV40- contaminated polio vaccine during the neonatal period” (Br J Cancer, Nov. 2001) study obviously looked at a cohort of only up to 35 years old, as you can deduce from the title. What’s more, there was an increase in testicular cancer warranting further investigation and monitoring:

4 deaths from cancer were found compared to 3.16 expected (P= 0.77). However, 2 deaths from testicular cancer occurred, compared to 0.05 expected (P= 0.002), which may be a chance finding due to multiple comparisons. There were 2 deaths due to leukaemia, a non-significant finding, and no deaths due to tumours of the types putatively associated with SV40. Although these results are, for the most part, consistent with other negative epidemiologic investigations of risks from SV40-contaminated vaccines, further study of testicular cancer may be warranted, and it will be important to continue monitoring this cohort which is now reaching middle-age.

Also:

Although large epidemiologic studies have not identified an elevated cancer risk in persons who received SV40-contaminated vaccines, fragments of SV40 DNA have recently been identified in certain human tumours.

To add to the cancer scare, “Emergent Human Pathogen Simian Virus 40 and Its Role in Cancer” (Clin Microbiol Rev. 2004 Jul) states:

A meta-analysis of molecular, pathological, and clinical data from 1,793 cancer patients indicates that there is a significant excess risk of SV40 associated with human primary brain cancers, primary bone cancers, malignant mesothelioma, and non-Hodgkin's lymphoma. Experimental data strongly suggest that SV40 may be functionally important in the development of some of those human malignancies. Therefore, the major types of tumors induced by SV40 in laboratory animals are the same as those human malignancies found to contain SV40 markers. The Institute of Medicine recently concluded that “the biological evidence is of moderate strength that SV40 exposure could lead to cancer in humans under natural conditions.” This review analyzes the accumulating data that indicate that SV40 is a pathogen which has a possible etiologic role in human malignancies. Future research directions are considered.

So when they tell you all is under control, that’s when you run.

Back to SV40 in the Covid jabs. As it turns out, the situation is very different from the SV40 in the polio jabs. In fact, there in no SV40 virus in the jabs at all, as this AP factcheck helpfully points out “No, ‘monkey virus DNA’ was not found in COVID vaccines” (AP, June 15, 2023).

I concur, as the original whistle-blower study “Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose” (OSFPREPRINTS, 2023.04.23) instead found the complete DNA plasmids in the jabs (both Moderna and Pfizer) that have been used in the production of the vaxx mRNA. These plasmids contained the S spike code, but in Pfizer jabs they also contained the chunk of SV40 called an “SV40 promoter”:

What is a promoter, you ask?

A promoter, as related to genomics, is a region of DNA upstream of a gene where relevant proteins (such as RNA polymerase and transcription factors) bind to initiate transcription of that gene. The resulting transcription produces an RNA molecule (such as mRNA).

SV40 promoter is about 317bp long, but the sequencing of the Pfizer plasmids also detected a repeat of the SV40 enhancer (72bp) at the tail of the SV40 promoter, in one of two Pfizer vials studied. As is well known, the 72bp SV40 enhancer is associated with more robust expression and nuclear localization. Sounds benign, right? Wrong! “The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants” (Nucleic Acids Res. 1981 Nov 25):

By introduction of recombinant plasmids into monkey CV1 cells, we have unambiguously demonstrated that sequences entirely within the 72bp repeat, which is located upstream of the SV40 early region, are crucial for T-antigen expression in vivo. We have also shown that a DNA fragment containing the 72bp repeat, inserted directly before chicken conalbumin or adenovirus-2 major late promoter sequences in chimeric plasmids where these promoters replace that of the SV40 early genes, caused a dramatic increase in the expression of T-antigen in vivo.

Two enhancers better than one, eh? SV40 on steroids, so to say. Now to nuclear localization:

A nuclear localization signal or sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport.

Indeed, “The role of plasmid constructs containing the SV40 DNA nuclear-targeting sequence in cationic lipid-mediated DNA delivery” (Cell Mol Biol Lett. 2005):

One of the steps that limit transfection efficiency in non-viral gene delivery is inefficient nuclear import of plasmid DNA, once it has been delivered into the cytoplasm. Recently, via microinjection into the cytoplasm and in situ hybridizations into a few cell types, it was shown that a region of Simian virus 40(SV40), specifically a c. 372-bp fragment of SV40 genomic DNA encompassing the SV40 promoter-enhancer-origin of replication (SV40 DTS), could enable the nuclear import of a plasmid carrying these sequences (Dean D.A. Exp. Cell Res. 230 (1997) 293).

So, we get 2-in-1 importation of the S spike-coding dsDNA plasmid into the cell nucleus and enhanced replication of the S spike in the transfected cell. What not to like! The authors of “Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose” state impartially:

There has been a healthy debate about the capacity for SARs-CoV-2 to integrate into the human genome(Zhang et al. 2021). This work has inspired questions regarding the capacity for the mRNA vaccines to also genome integrate. Such an event would require LINE-1 driven reverse transcription of the mRNA into DNA as described by Alden et al. (Alden et al. 2022). dsDNA contamination of sequence encoding the spike protein wouldn’t require LINE-1 for Reverse Transcription and the presence of an SV40 nuclear localization signal in Pfizer’s vaccine vector would further increase the odds of integration. This work does not present evidence of genome integration but does underscore that LINE-1 activity is not required given the dsDNA levels in these vaccines.

The nuclear localization of these vectors should also be verified.

So, as the integration of S spike code in the human genome has been strongly suspected before, it is now exceedingly likely that this, in fact, happens with dsDNA-contaminated jabs.

Contaminated how much, you ask? The authors of this study, “Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose” discovered that:

Multiple assays support DNA contamination that exceeds the European Medicines Agency (EMA) 330ng/mg requirement and the FDAs 10ng/dose requirements.

Agilent Tape Station™ electrophoresis demonstrates 23.7ng/µl – 55.9ng/µl of RNA. 7.5ng-11.3ng/µl are observed on DNA based Tape Station™.

Which means, 15-30% of contaminant DNA in the “mRNA” jabs. It’s in the title, actually. A microgram of dsDNA per dose is 100 times more than the explicit 10ng/dose FDA requirement. Not that anyone was, or is checking. As the current study covered the bivalent jabs also. Why FDA wanted to limit the DNA “garbage” in the jabs? Well,

Residual injected DNA can result in type I interferon responses and can increase the potential for DNA integration (Ulrich-Lewis et al. 2022).

As Wikipedia says:

The type-I interferons (IFN) are cytokines which play essential roles in inflammation, immunoregulation, tumor cells recognition, and T-cell responses.

So, you might just get the suppressed tumor cell recognition as a result of jabbing with the tainted Covid jabs, on top of everything else we’ve just covered.

Now prebook your fall jab with the updated Covid vaccine. Trust the Science!

P.S. A comment by TMartini67:

Here's some articles about when SV40 was used (and then covered up) in the early polio vaccines. No wonder there was an explosion of cancers ever since then.

• “The Virus and the Vaccine” (Atlantic Monthly, 2000)

• “New documents show the monkey virus is present in more recent polio vaccine” (SFGate. 2021.07.22)

• “SV40 (polio vaccines) - References”